Female mice had significantly higher IgG titers with higher variation compared to male mice. The effects of Immulix and/or increased fat content were minor. Immulix negatively affected IgG titers in male mice four weeks after secondary vaccination but upregulated Il1b gene expression in the spleen. Immulix had a downregulating effect on expression of Cd4 and Foxp3 in ileum only if the mice were fed the diet with increased fat. The diet with increased dietary fat increased Il1b but decreased Cd8a gene expression in the spleen. Immulix and diet affected GM composition significantly. Increased dietary fat content upregulated Lactobacillus animalis but downregulated an unclassified Prevotella spp. Immulix decreased Lactobacillales, Streptococcaceae and Prevotellaceae but increased Bacteroides.
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The family Alcedinidae was the most abundance (48%), was followed by Rhipiduridae (16%), Pycnonotiidae (12%) and Timaliidae (12%). The results of the study shows nine families of birds occurring in the arboretum, of which seven families were diurnal birds and two families were nocturnal birds. The results of this study show that the vegetation at the proposed arboretum is naturally regenerated acacia forest. Captured birds were identified, measured according to standard methods and ring banded. Mist netting was employed the bird survey to capture diurnal and nocturnal birds. Trees were identified up to species level. The vegetation survey was conducted using sampling plot that enumerates trees with DHB 10cm and above. Vegetation survey and bird surveys were conducted during this study. It elapsed from the 19 February to 14 March 2014 for the period of 24 days of survey. 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